Fragmentation of proteins in the 13- to 29-kDa mass range observed by 252Cf-plasma desorption mass spectrometry.
نویسندگان
چکیده
منابع مشابه
Laser desorption and matrix-assisted laser desorption/ionization mass spectrometry of 29-kDa Au:SR cluster compounds.
Positive and negative ions generated by laser-based ionization methods from three gold:thiolate cluster compounds are mass analyzed by time-of-flight mass spectrometry. The three compounds have similar inorganic core masses ( approximately 29 kDa, approximately 145 Au atoms) but different n-alkanethiolate ligands associated with each cluster compound (Au:SR, R = butane, hexane, dodecane). Irrad...
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252Cf plasma desorption mass spectrometry has been used in the characterization of more than 100 synthetic porphyrins ranging in mass from 614 u for tetraphenylporphyrin to over 2000 u for some porphyrin model systems. In virtually every case, 252Cf plasma desorption mass spectrometry yielded an intense ionized molecule ion [M.+ and/or (M+H)+], irrespective of the groups appended to the porphyr...
متن کاملProgress in plasma desorption mass spectrometry of peptides and proteins.
The principles and some applications of plasma desorption (PD) m.s. were described in this Journal in a report from the Biochemical Society's 618th Meeting in Liverpool on 2-4 April 1986 (Roepstorff et al., 1 9 8 7 ~ ) . Since then, PD-m.s. has developed to become a routine method in protein studies in our laboratory. During this period the sample preparation techniques using adsorption to nitr...
متن کاملAnalysis of unmodified endotoxin preparations by 252Cf plasma desorption mass spectrometry. Determination of molecular masses of the constituent native lipopolysaccharides.
Nine unmodified endotoxin preparations constituted of Re-, Rd-, and Rc-type lipopolysaccharides (2 to 5 glycoses), representing four species of enterobacteria were analyzed by 252Cf plasma desorption mass spectrometry. The constituent lipopolysaccharides were characterized by the ion pair: (M-H)- and its corresponding lipid fragment ion. The lipid fragment ion is produced by cleavage of the gly...
متن کاملAnalysis of the Photo Conversion of Asphaltenes Using Laser Desorption Ionization Mass Spectrometry: Fragmentation, Ring Fusion, and Fullerene Formation
The conversion or photo conversion of asphaltenes to polycyclic aromatic hydrocarbons (PAH’s) promoted by a laser source is analyzed using both experimental and theoretical methods. We propose that during measurements performed at an intermediate laser power, fragmentation to afford PAH’s and ring fusion to yield fused PAH’s (FPAH’s) may occur either within molecular clusters (resin case) or wi...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1992
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.89.13.6215